Revista Chapingo Serie Horticultura
Universidad Autónoma Chapingo
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Vol.20, núm.2 - 2014

Coffea canephora (Pierre) ex Froehner inoculado con micorriza y bacteria fijadora de nitrógeno en vivero
Coffea canephora (Pierre) ex Froehner inoculado con micorriza y bacteria fijadora de nitrógeno en vivero

José Carlos Ibarra-Puón; Juan Francisco Aguirre-Medina; Alejandro Ley-De Coss; Jorge Cadena-Iñiguez; Guillermo Armando Zavala-Mata

Recibido: 12-09-2013

Aceptado: 07-08-2014

Fecha de publicación en línea: 2014-08-15 /pág.201-213


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  • descriptionAbstract

    This study was conducted from November 2012 to May 2013 to evaluate the effect of inoculating C. canephora with Rhizophagus intraradices and / or Azospirillum brasilense in two substrates in nursery on some morphological and physiological yield components and to determine the amount of phosphorus in plant tissue and quantify mycorrhizal colonization. Soil used was mollic Andosol and two prepared substrates: one was soil and river sand and the other was soil plus 30% coffee pulp (1:1 v / v) in five-kg capacity plastic bags. Treatments were the control, Rhizophagus intraradices, Azospirillum brasilense and a combination of the two on each substrate, giving a total of eight treatments in a split plot in randomized block design with five replications. Samples were taken every 28 days to assess morphological and physiological variables and mycorrhizal colonization and phosphorus content during 140 days after transplant (dat). The results show that inoculation of C. canephora in nursery with either of the microorganisms individually favored growth and dry matter accumulation in morphological and physiological yield components relative to the control. A. brasilense promoted greater biomass accumulation during the evaluation and R. intraradices at the end of the study. R. intraradices increased phosphorus content in the tissue of plants, and higher mycorrhizal colonization was found in the first third of the root when inoculated with R. intraradices.

    Keyworks: Robusta coffee, Rhizophagus intraradices, Azospirillum brasilense, yield components, phosphorus, mycorrhizal colonization.

  • beenhereReferences
    • AGUIRRE-MEDINA, J.F.; KOHASHI-SHIBATA, J. 2002. Componentes morfológicos y fisiológicos del rendimiento, dinámica de la colonización micorrízica y contenido de fósforo en frijol Phaseolus vulgaris L. Agricultura Técnica en México 28(1): 23-33.

    • AGUIRRE-MEDINA, J. F. 2006. Biofertilizantes microbianos: experiencias agronómicas del programa nacional del INIFAP en México. Instituto Nacional de Investigaciones Forestales, Agrícolas y Pecuarias. Tuxtla Chico, Chiapas, México. 201p.

    • AGUIRRE-MEDINA, J. F.; MENDOZA-LÓPEZ, A.; CADENAIÑIGUEZ CADENAIÑIGUEZ, J.; AVENDAÑO-ARRAZATE, C. 2007. La Biofertilización del cacao (Theobroma cacao) L. en vivero con (Azospirillum brasilense) Tarrand, Krieg et Döbereiner y (Glomus intraradices) Schenk et Smith. Interciencia 32(8): 1-6.

    • AGUIRRE-MEDINA, J. F.; MOROYOQUI-OVILLA, D. M.; MENDOZA-LÓPEZ, A.; CADENA-IÑIGUEZ, J.; AVENDAÑO- ARRAZATE, C. H.; AGUIRRE-CADENA, J. F. 2011. Hongo endomicorrízico y bacteria fijadora de Nitrógeno inoculadas a Coffea arábica en vivero. Agronomía Mesoamericana 22(1): 71-80.

    • ANDRADE, S. A. L.; MAZZAFERA, P.; SCHIAVINATO, M. A.; SILVEIRA, A. P. D. 2009. Arbuscular mycorrhizal association in coffee. Journal of Agricultural Science 147(2): 105-115.

    • ANÓNIMO. 2000. SAS/STAT user´s Guide: Ver 8.1 SAS Institute Inc. Cary NC.

    • AUGÉ, R. M. 2004. Arbuscular mycorrhizae and soil/plant water relations. Canadian Journal of Soil Science 84(4): 373–381.

    • BASHAN, Y.; DE-BASHAN, LUZ, E. 2010. Chapter Two-How the Plant Growth-Promoting Bacterium Azospirillum Promotes Plant Growth-A Critical Assessment. Advances in Agronomy 108: 77-136.

    • BHATTARAI, N.; BARAL, B.; SHRESTHA, G.; YAMI, K. D. 2011. Effect of mycorrhiza and rhizobium on Phaseolus vulgaris L. Scientific World 9(9): 66-69.

    • CHATTOPADHYAY, N.; SWAIN, S.; HORE, J. K. 2006. Response of Coffee Seedlings to Nitrogen Fixing Biofertilizers. Agricultural Science Digest 26(2): 103-106.

    • CUENCA, G.; CÁCERES, A.; OIRDOBRO, G.; HASMY, Z.; URDANETA, C. 2007. Las micorrizas arbusculares como alternativa para una agricultura sustentable en áreas tropica-les. Interciencia 32(1): 23-29.

    • DOBBELAERE, S.; VANDERLEYDEN, J.; OKÓN J. 2001. Plant growth-promoting effects of diazotrophs in the rhizosphere. Critical Reviews in Plant Sciences 22(2): 107-149.

    • DONG, L. Q; ZHANG, K. Q. 2006. Microbial control of plant-parasitic nematodes: a five-party Interaction. Plant and Soil 288(1-2): 31–45.

    • DANIELL, T.; HUSBAND, J. R.; FITTER, A.H.; YOUNG, J.P.W. 2003. Molecular diversity of arbuscular mycorrhizal fungi colonizing arable crops. FEMS Microbiology Ecology 36(2- 3): 203-209.

    • GERDEMANN, J.W.; NICOLSON, T.H. 1963. Spores of mycorrhizal Endogone species extracted from the soil by wet sieving and decanting. Transactions of the British Mycological Society 46(2): 235-244.

    • GRANT, C.; BITTMAN, S.; MONTREAL, M.; PLENCHETTE, C.; MOREL, C. 2005. Soil and fertilizer phosphorus: Effects on plant P supply and mycorrhizal development. Canadian Journal of Plant Science 85(1): 3-14.

    • IRIZAR G., M. B.; VARGAS V., P.; GARZA G., D.; TUT C., C.; ROJAS M., I.; TRUJILLO C., A.; GARCÍA S., R.; AGUIRRE M., D.; MARTÍNEZ G., J. C.; ALVARADO M., S.; GRAJEDA C., O.; VALERO G., J.; AGUIRRE M., J. F. 2003. Respuesta de cultivos agrícolas a los biofertilizantes en la región central de México. Agricultura Técnica en México 29(2): 213- 225.

    • HARRIER, L. A.; WATSON, C. A. 2004. The potential role of arbuscular mycorrhizal (AM) fungi in the bioprotection of plants against soil-borne pathogens in organic and/or other sustainable farming systems. Pest Management Science 60(2): 149–157.

    • HERNÁNDEZ, W.; SALAS, E. 2009. La Inoculación con Glomus fasciculatum en el crecimiento de cuatro especies forestales en vivero y campo. Agronomía Costarricense 33(1): 17-30.

    • HUNGRIA, M.; CAMPO, R. J.; SOUZA, E. M.; PEDROSA, F. O. 2004. Inoculation with selected strains of Azospirillum brasilense and A. lipoferum improves yields of maize and wheat in Brazil. Plant Soil 331(1-2): 413-425.

    • KENNEDY, A. C. 2005. Rhizosphere, pp. 242-262. In: Principles and Applications of Soil Microbiology. SYLVIA, M. D.; FUHRMANN, J. J.; HARTE, G. P.; ZUBERER, A. D. (eds.). Second Edition. Pearson Prentice Hall. New Jersey, USA.

    • LEBRON, L.; LODGE, D. J.; BAYMAN, P. 2012. Differences in arbuscular mycorrhizal fungi among three coffee cultivars in Puerto Rico. ISRN Agronomy 2012: 1-7.

    • MUDGE, S. R.; SMITH, F. W.; RICHARDSON, A. E. 2003. Rootspecific and phosphate-regulated expression of phytase under the control of a phosphate transporter promoter enables Arabidopsis to grow on phytate as a sole P source. Plant Science 165(4): 871-878.

    • NGAKOU, A.; NGO N., L.; DOLOUM, G.; ADAMOU, S. 2012. Mycorrhiza-Rhizobium-Vigna subterranea dual symbiosis: Impact of microbial symbionts for growth and sustainable yield improvement. International Journal of Agriculture and Biology 14(6): 915-921.

    • PÉREZ, A.; BUSTAMANTE, C.; RODRÍGUEZ, R.; DÍAZ, A.; BERTOT, M.; RODRÍGUEZ, I. 2002. Influencia de diferentes variantes de fertilización en el crecimiento y desarrollo de posturas de Coffea canephora Pierre. Cultivos Tropicales 23(4): 89-93.

    • PHILLIPS, J. M.; HAYMAN, D. S. 1970. Improved procedures for clearing and staining parasitic and vesicular-arbuscular mycorrhizal fungi for rapid assessment of infection. Transactions of the British Mycological Society 55(1): 158-161.

    • RILLIG, M. C.; MUMMEY, D. L. 2006. Mycorrhizas and soil structure. New Phytologist 171(1): 41-53.

    • SERRALDE O., A. M.; RAMÍREZ G., M. M. 2004. Análisis de poblaciones de micorrizas en maíz Zea mays cultivado en suelos ácidos bajo diferentes tratamientos agronómicos. Revista Corpoica 5(1): 31-40.

    • SIEVERDING, E. 1989. Ecology of VAM fungi in tropical agrosystems. Agriculture, Ecosystems & Environment 29(1-4): 369-390.

    • SIQUEIRA, J. O.; COLOZZI-FILHO, A.; SAGGIN-JUNIOR, O. J.; GUIMARAES, P. T.G.; OLIVEIRA, E. 1993. Crescimento de mudas e producao do cafeeiro sob influencia de fungos micorrızicos e superfosfato. Revista Brasileira de Ciencia do Solo 17(1): 53–60.

    • SMITH, S. E.; SMITH, F. A.; JAKOBSEN, I. 2003. Mycorrhizal fungi can dominate phosphate supply to plant irrespective of growth responses. Plant Physiology 133(1): 16-20.

    • SYLVIA, M. D. 2005. Mycorrhizal symbioses, pp. 263-282. In: Principles and Applications of Soil Microbiology. SYLVIA, M. D.; FUHRMANN, J. J.; HARTE, G. P.; ZUBERER, A. D. (eds.). Second Edition. Pearson Prentice Hall. New Jersey, USA.

    • TAJINI, F.; DREVON, J. J. 2012. Phosphorus use efficiency in common bean (Phaseolus vulgaris L.) as related to compatibility of association among arbuscular mycorrhizal fungi and rhizobia. African Journal of Biotechnology 11(58): 12173- 12182.

    • TRISTÃO, F. S. M.; LOPEZ A., S. A.; SILVEIRA, A. P. D. 2006. Fungos micorrízicos arbusculares na formação de mudas de cafeeiro, em substratos orgânicos comerciais. Bragantia 65(4): 649-658.

    • WRIGHT, D. P.; SCHOLES, J. D.; READ, D. J.; ROLFE, S.A. 2005. European and African maize cultivars differ in their physiological and molecular responses to mycorrhizal infection. New Phytologist 167(3): 881–896.

  • starCite article

    Ibarra-Puón, J. C.,  Aguirre-Medina, J. F.,  Ley-De Coss, A., Cadena-Iñiguez, J., &  Zavala-Mata, G. A. (2014).  Coffea canephora (Pierre) ex Froehner inoculado con micorriza y bacteria fijadora de nitrógeno en vivero. , 20(2), 201-213.

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