Revista Chapingo Serie Ciencias Forestales y del Ambiente
Universidad Autónoma Chapingo
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Revista Chapingo Serie Ciencias Forestales y del Ambiente
Volume XXI, issue 1, January - April 2015
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Bioprospección de bacterias oxidantes de arsenito de suelo de la Comarca Lagunera
Bioprospecting arsenite oxidizing bacteria in the soil of the Comarca Lagunera

Edelweiss A Rangel-Montoya; Nagamani Balagurusamy

http://dx.doi.org/10.5154/r.rchscfa.2014.05.024

Received: 2014-04-29

Accepted: 2015-01-30

Available online: 2015-04-04 / pages.41–56

 

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  • descriptionAbstract

    Arsenic is one of the most toxic metalloids present in the environment and prolonged exposure to this metal causes chronic health effects. Therefore, the search for environmentally-friendly alternatives for the treatment of arsenic-contaminated water and soil is important. In this study, bacterial strains were isolated from arseniccontaining soils in the Lagunera region to analyze those with arsenite-oxidizing abiliity. Strains 04-SP1qa and 14-SP1qh with chemolithoautotrophic and chemoheterotrophic metabolism, respectively, had greater activity of the arsenite oxidase enzyme. The optimum growth conditions and enzymatic activity of these strains were investigated. Strain 04-SP1qa had specific enzymatic activity of 0.162 μmol·min-1·mg-1, Michaelis-Menten constant (Km) of 3.37 μM and maximum velocity (Vmax) of 5.2 μM·min-1·mg-1 under optimum growth conditions of pH 8.0 at 40 °C. Strain 14-SP1qh showed specific enzymatic activity of 0.16 μmol·min-1·mg-1, Km of 3.7 μM and Vmax of 14.39 μM·min-1·mg-1 at pH 7.0 and 40 °C. Results of this study demonstrated the presence of arsenite- oxidizing bacteria with enzymatic activity in the soils of the Lagunera region. Thus, the potential exists to develop new bioremediation technologies for treatment of arsenic-contaminated water and soils in the region using native bacterial strains.

    Keyworks: Arsenite oxidase, chemolithoautotrophic, chemoheterotrophic, bioremediation.
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  • starCite article

    Rangel-Montoya, E. A.,  &  Balagurusamy, N. (2015).  Bioprospecting arsenite oxidizing bacteria in the soil of the Comarca Lagunera. Revista Chapingo Serie Ciencias Forestales y del Ambiente, XXI(1), 41–56. http://dx.doi.org/10.5154/r.rchscfa.2014.05.024